Make sure your graph has a good title and you have labeled the axes, units, etc. Force the graph through zero. Use scatter chart (no lines) and add the trend line, equation, and R- squared value. (18 pts.) Prepare a standard curve of absorbance versus glucose concentration using Excel or another graphing program.
Standard Concentration Curve On Excel Mac If YouA nice feature of the functions in Excel is that once you select them and start typing, a small box will pop up underneath, which will give you the format you need to use with that particular function. The intensity of the colored reaction product is a direct function of protein amount that can be determined by comparing its absorbance value to a standard curve.(10.25 MB) Download How to draw standard curve in excel standard curve absorbance vs concentration excel AS Research MP3 & MP4 In this video.Now that we know how to correctly reference and fill in our calculations in Excel, it’s time to get started with some built-in functions. Get CalculatingUsing Microsoft Excel to plot and apply standard curve A protein assay, such the BCA Protein Assay, is an excellent tool for estimating the protein concentration of a sample. This time we’re going to move on to using some built-in Excel functions and go through how to apply all that we’ve done so far to a worked example that is relevant to the lab. Note: This feature is available on Windows or Mac if you have Office 2019.Last time we brushed up on cell referencing and constructing formulae to use Excel for some basic and some slightly more advanced calculations.![]() Insert a comma, the next value is for calculating where your line intercepts the axis, you can set this to 0, or you can leave it blank and allow Excel to determine the best point of intercept. Insert a comma and highlight your X values (in this case the standards in yellow) Highlight your Y values (in this case the average absorbance values in blue) Download monoca font for osxHit Ctrl + Shift + Enter (or Cmd + Enter for Mac) and your stats will appear! Sadly they don’t appear with labels so you’ll need to keep a copy of the explainer list handy, shown below.Now that you can sum, average and linest with the best of them, let’s try putting everything together into a worked example – calculating the concentration of an unknown protein from a standard curve.The warning icon will appear because we haven’t included every number in the row – that’s because we don’t want to average the value of our standard so it’s OK to ignore this!Using absolute referencing, subtract the blank value from your other standards.Now have a quick look at your standard deviation to make sure your values are not too varied! Go to the formula bar and click into the formula so that the X and Y values in the formula are highlighted Select the cell that contains your linest formula and drag the selection to include the cell to its right and down four rows Then type in “true”, or if you don’t need that sort of thing you can type “false” or leave it blank, just close the bracket and hit enter.For you eager beavers looking for additional statistics If you want values like your intercept, the standard error of intercept/slope/Y values, degrees of freedom, sum of square for regression etc. The final part of the function is about statistics. Right click on your trendline and select “Format trendline”5. Add a trendline by selecting one of the data point and right clicking.Now that you have a graph, you can add in the equation of the line Go to the “Insert” tab and insert and XY scatter plot.3. ![]()
0 Comments
Leave a Reply. |
AuthorDana ArchivesCategories |